Analytics
Six assays run on every lot before it is released. Below is what each one measures, the instrument that measures it, and — the part suppliers usually leave out — what it is structurally incapable of catching.
Agilent 1260 Infinity II, DAD @ 214 nm
Catches
Deletion sequences, truncations, oxidised residues, isomers
Blind to
Anything without a chromophore at 214 nm
Bruker amaZon SL ion trap
Catches
Wrong sequence, missing or extra residues, failed capping
Blind to
Isobaric substitutions such as Leu for Ile
Elemental nitrogen determination
Catches
Counterion and water inflating the apparent mass
Blind to
Non-nitrogenous excipients, were any present
Metrohm 899 coulometer
Catches
Incomplete lyophilisation, moisture ingress in transit
Blind to
Solvent other than water
Ion chromatography, suppressed conductivity
Catches
Trifluoroacetate carried through from purification
Blind to
Acetate and other exchanged counterions
Kinetic chromogenic LAL
Catches
Gram-negative pyrogen load from water or glassware
Blind to
Non-endotoxin pyrogens
Documentation
A single sheet, printed the hour the vials were sealed and packed flat against the carton wall. It carries the lot number on your label, the raw chromatogram, the deconvoluted mass, and the initials of whoever ran the sample.
The same PDF is available before you order. Ask for it — a supplier who hesitates at that request has told you something useful.
Request a certificateGpharmaBio
RESEARCH PEPTIDE LAB
CERTIFICATE OF ANALYSIS
GPB-4471 / REV 1
| TEST | SPECIFICATION | RESULT |
|---|---|---|
| Purity by RP-HPLC | ≥ 98.0% | 99.1% |
| Identity by ESI-MS | Conforms | Conforms |
| Net peptide content | Report | 82.4% |
| Water, Karl Fischer | ≤ 6.0% | 3.1% |
| Residual TFA | ≤ 1.0% | 0.19% |
| Endotoxin, LAL | ≤ 1.0 EU/mg | < 0.12 |
House rules
Certificates are generated per lot and stored against the lot number. If you ask for a certificate before ordering, you get the one for the material that will actually ship. Nobody here has the ability to send a representative trace, because we do not keep any.
A batch that misses specification is not diluted into a passing one. It goes to waste with the reason recorded, and the affected campaign is repeated. This is the single most expensive policy we have and the only one that is not negotiable.
We publish what each assay cannot see alongside what it can. A purity figure quoted without its method and detection wavelength is decoration, and we would rather hand you a slightly less flattering number you can actually interpret.